Parallel versus Off-Pathway Michaelis–Menten Mechanism for Single-Enzyme Kinetics of a Fluctuating Enzyme
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چکیده
منابع مشابه
Parallel versus Off-Pathway Michaelis-Menten Mechanism for Single-Enzyme Kinetics of a Fluctuating Enzyme.
Recent fluorescence spectroscopy measurements of the turnover time distribution of single-enzyme turnover kinetics of β-galactosidase provide evidence of Michaelis-Menten kinetics at low substrate concentration. However, at high substrate concentrations, the dimensionless variance of the turnover time distribution shows systematic deviations from the Michaelis-Menten prediction. This difference...
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ژورنال
عنوان ژورنال: The Journal of Physical Chemistry B
سال: 2015
ISSN: 1520-6106,1520-5207
DOI: 10.1021/acs.jpcb.5b03752